What's Warming Rate?
Shirley Pan, Xiaotian Deng
2018-06-28 00:50:43 in A Brief Overview of Cryopreservation
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Historically being considered as a less important factor, warming rate can have significant impact on the viability of cells. A number of researches show that the choice of warming rate shall not be independent from cooling rate, i.e. each cooling rate will result in a different optimal warming rate[1] [2]. One can determine the appropriate cooling rate warming rate combination only after carefully study the cooling/warming rate matrix.
[1] Farrant J., Lee H., Walter C. A.(1977). Effects of interactions between cooling and rewarming conditions on survival of cells The Freezing of Mammalian Embryos, pp. 49-67
[2] Farrant J., Walter C. A., Lee H., McGann L. E.(1977). Use of two step cooling procedures to examine factors influencing cell survival following freezing and thawing on survival of cells Cryobiology, 14:273-286